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. 2021 May 11;22(10):5083. doi: 10.3390/ijms22105083

Figure 6.

Figure 6

Immunohistochemical analysis of rod and cone photoreceptors in wild-type and conditional Exoc5 knockout mice at 20 weeks of age. Levels and localization of rhodopsin (green, Rho, (A)), red/green cone opsins (green, R/G Opsin, (B)), and RPE65 (green, RPE65, (C)) were assessed using immunohistochemistry in retinal sections of 20-weeks-old mice to identify alterations in rod and cone outer segments, as well as retinal pigment epithelium (RPE). Only moderate differences in staining were observed. Scale bars = 50 μm (A–C). Image J was used to quantify rhodopsin (A’) and cone opsin (B’) fluorescence in photoreceptors, and RPE65 (C’) in RPE of WT and Exoc5fl/fl;Best1-Cre+ mice. OS, outer segments; IS, inner segments; ONL, outer nuclear layer, INL, inner nuclear layer; RPE, retinal pigmented epithelium. ** p < 0.05 (WT vs. Exoc5fl/fl;Best1-Cre+ mutants).