Fig. 1.

v2H biosensors express more tau-fusion protein. Fluorescent micrographs of P301S v1 (a, d), v2L (b, e), and v2H (c, f) cells demonstrate an increase in fluorescence of both v2 cell lines over first-generation biosensor cells, and of v2H over v2L when examined under the YFP channel. d–f show cells treated with synthetic tau fibrils delivered by Lipofectamine 2000. Western blot against tau (g) and GFP (h) demonstrates higher levels of tau-fusion protein in v2H biosensor cells compared to v2L cells. Negative control (−) represents lysate from naïve HEK293 cells and positive control ( +) represents lysate HEK293 cells with a single incorporation of the tau RD-mClover construct. The slightly higher molecular weight of the tau-YFP fusion in v1 cells is due to a longer linking sequence in the original fusion protein