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RdDM is influenced by trigger–target sequence identity. (A) Alignment of PSTVd333 and TASVd361, showing the three regions of >24 nt sequence identity. Asterisks denote sequence consensus. (B) Crossing of Nt-di-TASVd361 with Nt-di-PSTVd333 leads to an abundant accumulation of TASVd siRNAs that map onto only three PSTVd333 sites. However, PSTVd333 cDNA was methylated almost at its full size, despite the absence of siRNAs.
