Table 2.
Real-time qPCR primers and probes for the target virus and the whole process control.
| Target gene fragment | Primer/probe | Final concentration (nM) | 5′ | Sequence | 3′ |
|---|---|---|---|---|---|
| SARS-CoV-2 | |||||
| Nucleocapsid (N1) | 2019-nCoV_N1-F | 200 | None | GACCCCAAAATCAGCGAAAT | None |
| 2019-nCoV_N1-R | 200 | None | TCTGGTTACTGCCAGTTGAATCTG | None | |
| 2019-nCoV_N1-P | 200 | FAM | ACCCCGCATTACGTTTGGTGGACC | /ZEN//3IaBkFQ/ | |
| Nucleocapsid (N2) | 2019-nCoV_N2-F | 200 | None | TTACAAACATTGGCCGCAAA | None |
| 2019-nCoV_N2-R | 200 | None | GCGCGACATTCCGAAGAA | None | |
| 2019-nCoV_N2-P | 200 | FAM | ACAATTTGCCCCCAGCGCTTCAG | /ZEN//3IaBkFQ/ | |
| Nucleocapsid (N3) | 2019-nCoV_N3-F | 200 | None | GGGAGCCTTGAATACACCAAAA | None |
| 2019-nCoV_N3-R | 200 | None | TGTAGCACGATTGCAGCATTG | None | |
| 2019-nCoV_N3-P | 200 | FAM | AYCACATTGGCACCCGCAATCCTG | /ZEN//3IaBkFQ/ | |
| Envelope (E) | E_Sarbeco_F | 400 | None | ACAGGTACGTTAATAGTTAATAGCGT | None |
| E_Sarbeco_R | 400 | None | ATATTGCAGCAGTACGCACACA | None | |
| E_Sarbeco_P1 | 200 | FAM | ACACTAGCCATCCTTACTGCGCTTCG | /ZEN//3IaBkFQ/ | |
| PRCV | |||||
| PRCV | PRCV_1_F | 200 | None | AGCTATTGGACTTCAAAGGAAGATG | None |
| PRCV_1_R | 200 | None | CATAGGCATTAATCTGCTGAAGGAA | None | |
| PRCV_1_P | 100 | HEX | TCACGTTCACACACAAATACCACTTGCCA | /ZEN//3IaBkFQ/ | |