Table 3.
Comparison of qPCR and dPCR results for all gene fragments of interest for seven IWW samples collected in the tail of the second wave. CI = 95% confidence interval. Matrix composition (i.e. number of PCR inhibitors) was different between the different samples.
| Sample | Real-time qPCR |
Digital PCR |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| N1 (copies/μL) | N2 (copies/μL) | E (copies/μL) | N1 (copies/μL) | CI (%) | N2 (copies/μL) | CI (%) | E (copies/μL) | CI (%) | |
| 1 | 0.17 | 1.16 | n.d. | 0.70 | 95.7 | 0.54 | 109.1 | 0.27 | 168.6 |
| 2 | n.d. | n.d. | n.d. | 0.13 | 274.4 | n.d. | – | 0.26 | 168.6 |
| 3 | 0.46 | 1.15 | 10.17 | 1.33 | 64.9 | 1.00 | 79.0 | 0.13 | 274.4 |
| 4 | n.d. | 0.10 | 13.57 | n.d. | – | n.d. | – | 0.13 | 274.4 |
| 5 | 0.08 | 0.50 | n.d. | n.d. | – | n.d. | – | n.d. | – |
| 6 | 0.20 | n.d. | n.d. | 0.14 | 274.4 | n.d. | – | n.d. | – |
| 7 | 0.17 | 0.74 | 1.27 | 1.77 | 56.3 | 0.53 | 109.1 | 0.40 | 274.4 |