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. Author manuscript; available in PMC: 2021 Aug 21.
Published in final edited form as: ACS Chem Biol. 2020 Jul 28;15(8):2299–2310. doi: 10.1021/acschembio.0c00537

Figure 2.

Figure 2.

Generation and characterization of anti-DNP CAR-NK cells. (A) Schematic representation of the anti-DNP CAR construct. CD28TM: CD28 transmembrane domain. (B) Verification of anti-DNP CAR expression on NK-92MI cells after lentiviral transduction and magnetic cell sorting. CAR-NK cells were stained with an anti-HA-tag antibody and a PE-conjugated secondary antibody and then analyzed by flow cytometry. (C) Verification of the DNP-binding ability of CAR-NK cells by flow cytometry. Cells were stained with DNP-conjugated PE.