Skip to main content
. 2021 May 13;11(5):311. doi: 10.3390/metabo11050311

Figure 3.

Figure 3

Dependence of detected oxylipins on serum concentrations in the culture medium in LPS-stimulated cells, cultivated in normal (5.5 mM) or high (25 mM) glucose medium. Primary rat astrocytes were cultivated in normal (5.5 mM) or high (25 mM) glucose medium with FBS concentration at 10%, 5%, and 1%, and then stimulated with lipopolysaccharide (LPS, 100 ng/mL) for 4 h. Concentrations of oxylipins in supernatants were measured using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The values represent a mean ± SEM from three independent experiments. (A) the concentrations of the detected PUFAs were summed up; (B) the concentrations of the detected LOX-derived metabolites were summed up; (C) the concentrations of the detected COX-derived metabolites were summed up; (D) the results expressed as fold change, relative to the control, with the corresponding percentage of serum. * p < 0.05, compared with the LPS-stimulated cells under normal glucose conditions, with 10% FBS; # p < 0.05, compared with the LPS-stimulated cells under high glucose conditions, with 10% FBS. Abbreviations: PUFAs-polyunsaturated fatty acids, LOX-lipoxygenase, COX-cyclooxygenase, TXB2-thromboxane B2, HETE-hydroxyeicosatetraenoic acids, HDoHE-hydroxydocosahexaenoic acids, HHT-hydroxyheptadecatrenoic acid, 6-keto-PGF1a-6-keto-prostaglandin F1a, PGA2-prostaglandin A2, PGE2-prostaglandin E2, PGD2-prostaglandin D2, PGF2a-Prostaglandin F2a.