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Production of siderophore by the wild-type and T2 strain. (a) HPLC analysis of the culture supernatant of the wild-type (WT) or the transformant (T2) grown in a minimal medium containing 10 μM Fe3+. (b) HPLC analysis of the culture supernatant of the wild type (WT) or the transformant (T2) grown in minimal medium without iron. (c) Modified chrome azurol-S (CAS) assay for the detection of siderophore. The peak fraction (5 min) from the HPLC analysis was subjected to a CAS assay.
