Figure 4.

Effect of culture pH and temperature on the siderophore production and the mycelial growth of the wild-type (WT) and the transformant (T2). (a) Effect of pH. The strains were grown in C-PDB (50 mL) with different pH values for 2 weeks at 25 °C. (b) Effect of temperature. The strains were grown in C-PDB (50 mL) for 2 weeks at different temperatures. Siderophore in the culture supernatant was analyzed by a CAS assay, and the mycelial mass was measured after drying at 60 °C for 24 h. The siderophore productions shown here are the relative productions compared to the maximum values under the experimental conditions. Both experiments were carried out in triplicate, and the mean values are plotted with standard errors as error bars.