Figure 7.

Role of integrins in the initial adhesion of ADSCs and HUVECs to substrate-immobilized Gal-3 (1 h after seeding). (A) The cells were seeded in a medium containing EDTA in concentrations ranging from 0.1 to 10 mM. (B) The cells were seeded in the medium containing antibodies against the β1, β3, α3, and αV integrin subunits. The antibody against αV integrin subunit was used in a 1:25 dilution, and the other antibodies were used in a concentration of 20 μg/mL. Nonspecific mouse IgG1 was used as an isotype control. (C) The cells were seeded in the medium containing antibodies against α2β1, α5β1, or αVβ3 integrin receptors. Nonspecific mouse IgG1 was used as an isotype control. Antibodies were used in a concentration of 20 μg/mL. (D) The cells were seeded in a medium containing the αVβ1 inhibitor in concentrations ranging from 0.01 to 10 µM. (E) The cells were seeded in a medium containing the GRGDSP peptide. The GRADSP peptide was used as a negative control. Both peptides were used in a concentration of 200 µM. Before cell seeding, the wells were coated with 1 µM Gal-3 and blocked with 0.5% w/v BSA. The cell index values were normalized to the untreated sample in a pure medium without any additive (ctrl). Mean ± SD from 3 wells. Holm-Sidak test, p ≤ 0.05. The samples were statistically compared within the group of the indicated cell type. * Statistically significant difference in comparison with the control cells (ctrl). # Statistically significant difference in comparison with the cells incubated with the isotype control (IgG1).