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. 2021 May 26;12:299. doi: 10.1186/s13287-021-02363-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The intestinal barrier function was impaired after sepsis. a EB leakage of intestine at different times after sepsis (n=8). b Wet weight to dry weight ratio of intestine at different times after sepsis (n=8). c Blood D-lac level at different times after sepsis (n=8). d Blood TNFα level at different times after sepsis (n=8). e Blood Zonulin level at different times after sepsis (n=8). f Representative microphotographs of HE staining in intestine. The scale bar represents 100μm. g The relative expression of PCNA in intestine at different times after sepsis. β-actin was used as the internal reference (n=5). h TEER of IEC-6 after LPS stimulation (n=5). i BSA leakage of IEC-6 after LPS stimulation (n=6). j CCK8 proliferation assay of IEC-6 (n=6). k Representative microphotographs of the scratch migration assay and the scratched area were shown. The scale bar represents 100μm. l The relative expression of PCNA in IEC-6 after LPS stimulation. β-actin was used as the internal reference (n=5). m Representative IF microphotographs of ZO-1 in IEC-6 after LPS stimulation. The scale bar represents 20μm. n Representative IF microphotographs of Tunel assay in IEC-6. The scale bar represents 50μm. Ctl, normal control group; C8h, 8h after CLP group; C16h, 16 h after CLP group; C24h, 24h after CLP group. **P < 0.01, *P < 0.05