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. 2021 May 26;12:299. doi: 10.1186/s13287-021-02363-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — MMVs delivered functional mitochondria to IEC-6. a Flow cytometry analysis of mitochondria-positive MMVs detected by mitotracker-Red. b Representative TEM microphotographs of mitochondria-carried MMVs. The scale bar represents 500nm. c Flow cytometry analysis of ΔΨm in MMVs. d OCR analysis in MMVs (n=5). e Representative confocal microphotographs of the fusion of MMV-delivered mitochondria (Green pseudocolor) and the mitochondria in IEC-6 (Red pseudocolor). The scale bar represents 10μm. f The relative fluorescence intensity of MMVs and the co-location rate of two mitochondria in IEC-6 (n=5). g Flow cytometry analysis of mitochondria-positive MMVs in PGC-1α upregulated MMVs (n=5). **P < 0.01