Table 1.
CC50 values for vehicle (DMSO) and betulin
| cell line | DMSO (MTT) | betulin | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MTT | NRU | SRB | ||||||||||
| 24 h | 48 h | 72 h | 24 h | 48 h | 72 h | 24 h | 48 h | 72 h | 24 h | 48 h | 72 h | |
| NIH/3T3 |
2.989 ± 0.159 |
2.374 ± 0.395 |
1.635 ± 0.423 |
1.532 ± 0.005 |
1.585 ± 0.64 |
1.853 ± 0.647 |
˃15.625 |
7.329 ± 2.942 |
12.954 ± 2.942 |
10.87 ± 7.338 |
4.051 ± 1.242 |
3.756 ± 0.572 |
| BF-2 |
5.031 ± 0.423 |
4.434 ± 0.717 |
4.794 ± 0.337 |
3.394 ± 0.903 |
2.751 ± 1.051 |
3.021 ± 1.034 |
27.615 ± 9.006 |
16.462 ± 3.599 |
14.516 ± 3.41 |
>31.25 | >31.25 | >31.25 |
DMSO concentrations in %, betulin concentrations in µg/mL. CC50 (50 % cytotoxic concentration) values expressed as means ± SD (standard deviation) for n = 3 independent experiments. CC50 values were calculated using the Quest Graph™ ED50 Calculator (AAT Bioquest, Inc.)