Table 1.
Sequences of primers used in qPCR
| Gene | Primer | Sequence 5'–3' | Amplicon length (bp) | Accession No |
|---|---|---|---|---|
| Mfn1 |
F: R: |
GTTGCCGGGTGATAGTTGGA TGCCACCTTCATGTGTCTCC |
146 | NM_033540.3 |
| Mfn2 |
F: R: |
AATCTGAGGCGACTGGTGAC GGACATTGCGCTTCACCTTC | 126 | XM_024451299.1 |
| Parkin |
F: R: |
GTGCAGAGACCGTGGAGAAA GCTGCACTGTACCCTGAGTT | 294 | NM_013987.3 |
| Sod1 (cu/zn sod) |
F: R: |
CATTCCATCATTGGCCGCAC GAGCGATCCCAATCACACCA |
130 | NW_001867397.1 |
| Sod2 (mn sod) |
F: R: |
GGACAAACCTGAGCCCCAAT TTGGACACCAGCCGATACAG |
125 | NW_001867408.1 |
| Cat |
F: R: |
ACCAAGGTTTGGCCTCACAA TTGGGTCAAAGGCCAACTGT |
112 | XM_014851065.1 |
| GPx |
F: R: |
TCCGGGACTACACCCAGATG TCTTGGCGTTCTCCTGATGC |
108 | NM_000581.4 |
| TLR4 |
F: R: |
GACGGTGATAGCGAGCCAC TTAGGAACCACCTCCACGCAG |
173 | NM_138554.5 |
| Pink1 |
F: R: |
GCTTGGGACCTCTCTTGGAT CGAAGCCATCTTGAACACAA |
142 | NM_032409.3 |
| NF-kB-p65 |
F: R: |
CTGTTCCCCCTCATCTTCCC GTATCTGTGCTCCTCTCGCC |
113 | L19067.1 |
| c-Jun |
F: R: |
ACGGCGGTAAAGACCAGAAG CCAAGTTCAACAACCGGTGC | 89 | NM_002228.4 |
| IL-1β |
F: R: |
AAACAGATGAAGTGCTCCTTCCAG TGGAGAACACCACTTGTTGCTCCA |
391 | NM_000576.3 |
| IL-4 |
F: R: |
CTTTGCTGCCTCCAAGAACAC GCGAGTGTCCTTCTCATGGT |
97 | NM_000589.4 |
| IL-6 |
F: R: |
TCCTTCTCCACAAACATGTAACAA ATTTGTGGTTGGGTCAGGGG |
319 | NM_001318095.2 |
| TNF-α |
F: R: |
AGTGACAAGCCTGTAGCCCA GTCTGGTAGGAGACGGCGAT | 242 | NM_000594.4 |
| p53 |
F: R: |
AGATAGCGATGGTCTGGC TTGGGCAGTGCTCGCTTAGT |
381 | NM_001126118.1 |
| Insr |
F: R: |
TAGACGTCCCGTCAAATATTGC GAAGAAGCGTAAAGCGGTCC | 244 | AH002851.2 |
| Irs2 |
F: R: |
GAGCTGTGGCGTTTCACATC AGCTTCGGGCTGAAACAGT | 234 | NM_003749.3 |
| Akt1 |
F: R: |
CTGTCATCGAACGCACCT GTCTGGATGGCGGTTGTC | 178 | NM_005163.2 |
| Akt2 |
F: R: |
TCAAAGAAGGCTGGCTCCAC TGTACCCAATGAAGGAGCCG | 205 | M95936.1 |
| Pi3k |
F: R: |
TTTAATCTGCCAGGCGGAGG CCAGAATTCCATGGGGCAGT | 151 | NM_006218.4 |
| Bax |
F: R: |
ACCAAGAAGCTGAGCGAGTGTC ACAAAGATGGTCACGGTCTGCC |
356 | XM_011527191.1 |
| Bcl-2 |
F: R: |
ATCGCCCTGTGGATGACTGAG CAGCCAGGAGAAATCAAACAGAGG |
129 | NM_000633.2 |
| p21 |
F: R: |
AGAAGAGGCTGGTGGCTATTT CCCGCCATTAGCGCATCAC |
169 | NM_001220777.1 |
| Casp3 |
F: R: |
CTCTGGTTTTCGGTGGGTGT CTTCCATGTATGATCTTTGGTTCC |
136 | NM_004346.4 |
| Casp9 |
F: R: |
CAGGCCCCATATGATCGAGG CTGGCCTGTGTCCTCTAAGC |
142 | NM_032996.3 |
| Sirt1 |
F: R: |
TCACTGCACCACTTGAGTCC ACAGGTTGCGGGAATCCAAA | 73 | NM_012238.5 |
| Foxo1 |
F: R: |
CTCTGGCCCCTTTCACCATA GCTTTCTTCTTGGCAGCTCG | 229 | XM_011535010.2 |
| G6PC |
F: R: |
CACTTCCGTGCCCCTGATAA AGTATACACCTGCTGTGCCC | 95 | NM_000151.4 |
| GAPDH |
F: R: |
GTCAGTGGTGGACCTGACCT CACCACCCTGTTGCTGTAGC |
256 | NM_001289746.1 |
Mfn1, Mitofusin 1; Mfn2, Mitofusin 2; Parkin, Parkin RBR E3 ubiquitin protein ligase (PARK2); Pink1, PTEN-induced putative kinase 1; Sod1 (Cu/Zn SOD), Copper-zinc-dependant superoxide dismutase (CuZnSOD; Sod2 (Mn SOD), Manganese-dependent superoxide dismutase (MnSOD); CAT, Catalase; GPx, Glutathione Peroxidase; TLR4, Toll like receptor 4; NF-kB-p65, Nuclear Factor-kappa-B transcription factor p65; c-Jun, Jun Proto-Oncogene, AP-1 Transcription Factor Subunit; IL-1β, Interleukin 1 beta; IL-4, Interleukin 4; IL-6, Interleukin 6; TNF-α, Tumor Necrosis Factor alpha; P53, tumor suppressor p53; Insr, Insulin Receptor; Irs2, Insulin Receptor Substrate 2; Akt1, Serine/threonine 308 Kinase 1; Akt2, serine/threonine kinase 2; Pi3k, Phosphoinositide 3-Kinase; Bcl-2, B-cell lymphoma 2; Bax, BCl-2 associated X protein; p21, Cyclin-dependent kinase inhibitor 1; Casp3, Caspase 3; Casp9, Caspase 9; Sirt1, Sirtuin 1; Foxo1, forkhead box O1; G6PC, Glucose 6 phosphate Catalytic subunit; GADPH, Glyceraldehyde-3-phosphate dehydrogenase