Table 2.
List of antibodies employed for proteins profiling using western blot analysis
| Antibody | Dilution | Catalog No |
|---|---|---|
| Insr | 1:1000 | Invitrogen, MA1-10865 |
| Akt Pan | 1:1000 | Invitrogen, 44-609G |
| Pi3kCD | 1:1000 | Invitrogen, PA5-83748 |
| SIRT1- N-terminal region | 1:1000 | ARP32386 |
| TOR/mTOR | 1:500 | nb100-240 |
| NF-kappaB p65(phospho-T254) | 1:1000 | Biorbyt, orb304547 |
| JNK1/JNK2(phospho-Thr183/Tyr185) | 1:1000 | Biorbyt, orb15028 |
| Actin | 1:2000 | Sigma Aldrich, a2066 |
Insr, Insulin Receptor; Akt, Protein Kinase B; Pi3kCD, Phosphatidylinositol 3-Kinase; SIRT1, Sirtuin 1; TOR/wTOR, Target of rapamycin/Mammalian Target of rapamycin; NF-kappaB p65(phospho-T254), Phosphorylated Nuclear Factor-kappa-B transcription factor p65; JNK1/JNK2(phospho-Thr183/Tyr185), Phosphorylated c-Jun N-terminal kinase