Figure 8.

KLF5 was essential for the proliferation of gastric cancer cells. (A) KLF5 mRNA (RT-PCR) and protein (Western blot) expression in seven gastric cancer cell lines; (B) RT-PCR showing that KLF5 specific siRNA could downregulate KLF5 mRNA effectively in two gastric cancer cell lines; (C) Western blot confirmation of KLF5 downregulation in gastric cancer cell lines; (D) MTT assay showing that cell proliferation was significantly compromised after KLF5 was downregulated by siRNA; (E) the proliferation of gastric organoids was significantly reduced when KLF5 was downregulated by siRNA, as indicated by lower organoid formation efficiency and smaller organoid size. Abbreviations: RT-PCR, real-time PCR; CON-si, control siRNA. The scale bar indicated 100 μm.