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. 2021 May 22;8(3):ENEURO.0481-20.2021. doi: 10.1523/ENEURO.0481-20.2021

Figure 4.

Figure 4.

Shank3 S782 phosphorylation does not affect PDZ domain binding. A, myc-GKAP or Homer1b-myc were expressed in HEK293 cells and the cell lysates were incubated with GST-Shank3 fusion proteins (WT, S782A, or S782D) for pulldown assays. myc-GKAP or Homer1b-myc in the pulldown sample were analyzed by immunoblotting. B, GST-Shank3 fusion proteins (WT, S782A, or S782D) were incubated with rat brain P2 lysates for pulldown assays. The pulldown samples were analyzed by immunoblotting with indicated antibodies. C, HA-Shank3 (WT, S782A, or S782D) and myc-GKAP were cotransfected in HEK293 cells (left). HA-Shank3 and myc-GKAP were cotransfected with or without CaMKII in HEK293 cells (right). HA-Shank3 was immunoprecipitated with HA antibody and myc-GKAP was analyzed by immunoblotting. D, Quantitative graphs represent mean ± SEM (n = 3), and statistical significance was calculated using one-way ANOVA with Dunnett’s multiple comparison test (top) or an unpaired t test (bottom). n.s., not significant.