Supplementary Table 2.
Antibodies used in Western blot and immunohistochemistry
| Antibodies | WB | IHC | Product information |
|---|---|---|---|
| HIF-1α | 1:1000 | 1:100 | ab2185, Abcam, Cambridge, MA, USA |
| eNOS | 1:1000 | 1:100 | ab76198, Abcam |
| nNOS | ---- | 1:100 | Ab5586, Abcam |
| Bcl-2 | 1:800 | 1:80 | AF6139, Affinity Bioscience, Wuhan, China |
| Bax | 1:800 | 1:80 | AF0120, Affinity Bioscience |
| Caspase3 | 1:1000 | ---- | 9662S, Cell Signaling Technology, Framingham, MA, USA |
| Cleaved-caspase3 | 1:800 | 1:80 | AF7022, Affinity Bioscience |
| PI3K | 1:1000 | ---- | 4255S, Cell Signaling Technology |
| AKT | 1:1000 | ---- | 9272S, Cell Signaling Technology |
| Phospho-AKT | 1:1000 | ---- | 9271S, Cell Signaling Technology |
| mTOR | 1:1000 | ---- | 2983S, Cell Signaling Technology |
| Phospho-mTOR | 1:1000 | 1:80 | 5536S, Cell Signaling Technology |
| ULK1 | 1:800 | ---- | ET1704-63, HuaAn Biotechnology, Hangzhou, China |
| Phospho-ULK1 | 1:800 | 1:80 | AF7022, Affinity Bioscience |
| Beclin1 | 1:1000 | 1:80 | AF5128, Affinity Bioscience |
| p62 | 1:1000 | 1:80 | AF5384, Affinity Bioscience |
| LC3A/B | 1:1000 | 1:100 | 12741S, Cell Signaling Technology |
| β-actin | 1:2000 | ---- | M1210-2, HuaAn Biotechnology |
| Goat anti-rabbit IgG | 1:10000 | ---- | S0001, Affinity Bioscience |
| Goat anti-mouse IgG | 1:10000 | ---- | S0002, Affinity Bioscience |
PI3K: phosphatidylinositol 3-kinase; mTOR: mammalian target of rapamycin; ULK1: unc-51-like autophagy-activating kinase 1; ----, no relevant measurement; WB: western blot; IHC: immunohistochemistry; HIF-1α: hypoxia-inducible factor-1α; eNOS: endothelial nitric oxide synthesis; nnos: neuronal nitric oxide synthesis; Bcl-2: B-cell lymphoma-2; Bax: Bcl-2-associated X protein; AKT: protein kinase B.