Fig. 3. (A) SNV detection in KRAS wild type (KRAS-WT) vs. variants, -A, -T, and -C. Conditions: pH 7.4 1× PBS buffer, 0.01% Tween-20, 5 mM NaAsc, 25 °C, 50 nM of DC1-C, 5 nM of RuUD1 and RuDD1, 1 nM of targets, and irradiation with a 1 W LED (455 nm). Templated reaction has been done in the presence of an (B) additional salt concentration (1 h reaction) or (C) additional single stranded sperm DNA (1 h reaction) to evaluate condition robustness. (D) Detection of KRAS-T or -A in the presence of an excess amount of KRAS-WT sequences and 300 ng of sperm ssDNA. 1 or 2% of KRAS-T or KRAS-A was detectable in 5 nM total target concentration (1 h reaction). Statistics were obtained by an unpaired two-sample t-test (*p < 0.05).