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. 2021 May 19;41(20):4378–4391. doi: 10.1523/JNEUROSCI.2537-20.2021

Figure 4.

Figure 4.

MET currents in IHCs of control and p.T416K mutant mice. A, MET current for Tmc1+/+; Tmc2−/− in response to sinusoidal motion of hair bundle. B, MET current for Tmc1 p.T416K/T416K; Tmc2−/−. C, Collected results of MET current amplitudes in five control (black) and five p.T416K/T416K mutant (red) P8 IHCs. For A–C, holding potential −84 mV. D, Hair cell fluorescence in unfixed cochleas of P30 mice indicating FM1-43 entry in apical hair cells of Tmc1+/+; Tmc2−/− (top), and Tmc1 p.T416K/T416K; Tmc2−/− mice in the absence (middle) and presence (bottom) of 0.1 mm d-tubocurarine (curare) to block MET channels. In the mutant, OHCs are shrunken and some are missing. E, Collected results showing the fluorescence intensity (au) in 36 IHCs and 40 OHCs in two preparations. Tmc1+/+ and Tmc1 p.T416K were significantly different (***p < 0.001) for IHCs and OHCs but Tmc1 p.T416K with and without d-tubocurarine were not significantly different (p = 0.28 IHCs, p = 0.18 OHCs). Results indicate functional channels that can be blocked by curare in Tmc1+/+ but not Tmc1 p.T416K. In panels C and E, bars denote mean +/− SD.