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. 2021 May 19;41(20):4378–4391. doi: 10.1523/JNEUROSCI.2537-20.2021

Figure 9.

Figure 9.

Loss of mechanotransduction determined with FM1-43 influx. A, Fluorescence images showing one row of IHCs and three rows of OHCs in the apical turn of cochlear explants of P15 Tmc1 p.T416K/T416k; Tmc2−/− mice after exposure to 6 μm FM1-43 in the absence (top) and presence (bottom) of 0.1 mm curare. B, Fluorescence intensity in IHCs and OHCs with and without d-tubocurarine in P15 mice (left) and P21 mice (right). Number of cells measured (±curare): P15 IHCs 76, 65; P15 OHCs 120, 145; P21 IHCs 55, 50; OHCs 69, 72. C, Fluorescence images in apical turns of P15 Tmc1 p.D528N/D528N; Tmc2−/− mice after exposure to 6 μm FM1-43 in the absence (top) and presence (bottom) of 0.1 mm curare. D, Fluorescence intensity in IHCs and OHCs ± curare in P15 mice (left) and P21 mice (right). Number of cells measured (±curare): P15 IHCs 81, 80; P15 OHCs 278, 178; P21 IHCs 25, 24; OHCs 68, 61. The small number of P21 IHCs reflect loss of these cells. All IHC measurements were made at slightly different focal plane to OHCs; *** statistical significance indicated t test, p < 0.001, in panles B–D, bars denote mean +/− SD.