Figure 7.

Ablation of CnB in S63del Schwann cells in P28 sciatic nerves. A, WB for CnA and CnB was performed in P28 sciatic nerves from WT and CnB^SCKO mice. β-Tubulin (Tub) represents the control for loading. B, Quantification of the WB for CnA and CnB. Error bars indicate SEM. **p < 0.01 (Student's t test). n = 3. C, EM of P28 sciatic nerves of WT, S63del, S63del/CnB^SCKO, and S63del/CnB^SCKO/Perk^SCKO mice. Scale bar, 2 μm. Quantification of % of amyelinated axons (red arrowheads) (D) and g-ratio (E) was performed over a total number of 300-400 axons per animal using 3 or 4 animals per genotype, except for S63del/CnB^SCKO/Perk^SCKO for which we quantified 2 animals. Error bar indicates SEM. **p < 0.01; one-way ANOVA with Tukey's multiple comparison test. Although it is not shown, all the WT genotypes were statistically significant against the S63del genotypes in the quantifications of % of amyelinated axons, as previously reported (Sidoli et al., 2016).