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. 2021 May 12;9:639111. doi: 10.3389/fcell.2021.639111

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Copyright © 2021 Zhao, Fu, Hu, Chen, Wang, Fang, Ge, Lin, Pan, Fu, Wang, Du and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Short-term and long-term deprivations of serine have opposite effects on the regulation of YAP expression. (A) Immunoblotting analysis of YAP and p-YAP protein expression in SW620 and SW620-S, LOVO, and LOVO-S cells. (B,C) Analysis of cellular localization of YAP in SW620, SW620-S, LOVO, and LOVO-S cells. Immunoblotting analysis of YAP in Nuc. and Cyt. separation. Enolase 1 and H3 are markers for cytoplasm and nuclear, respectively. Cyt., Cytoplasm; Nuc., nucleus. (D) Immunoblotting analysis of YAP protein expression in SW620 and LOVO cultured under a medium with or without serine. (E) Nucleocytoplasmic separation analysis of YAP cellular localization change in SW620 cells cultured with or with serine contained medium for 24 h. Immunoblotting analysis of Nuc. and Cyt. separation with the indicated antibodies.