Skip to main content
. 2020 Jul 9;117(6):1592–1605. doi: 10.1093/cvr/cvaa201

Figure 4.

Figure 4

Glypican 1 silencing enhances EC dysfunction in cells cultured on soft 2.5 kPa gels, while having no effect on cells cultured on stiff 10 kPa gels. Cells cultured on 2.5 or 10 kPa polyacrylamide gels were treated with an siRNA to silence Glypican 1 expression; alternatively, cells were treated with a control, non-targeting scrambled siRNA, followed by assessment of cell function. (A) Inflammation was assessed by a monocyte adhesion assay and data were quantified as percentage bound monocytes on the surface of ECs, N = 5–6. (B) Expression of phospho-eNOS (green) was assessed by immunostaining, nuclei were stained with DAPI (blue) N = 4–5. Scale bar = 20 μm. (C) Inflammatory marker expression was assessed by qPCR, HPRT was used as a housekeeping gene N = 6. (D) Cell proliferation was assessed by ki67 (red) immunostaining and nuclei were stained with DAPI (blue). The data were quantified as percentage of cells positive for ki67, N = 5–6. (E) EndMT gene marker expression was assessed by qPCR, N = 7. Mean values ± S.E.M. are shown. *P < 0.05, **P < 0.01, and ***P < 0.001 using Student’s t-tests and ANOVA tests.