Figure 7.
The effect of PI3K blocking by specific siRNA (si) and subsequent temozolomide (T) incubation on the level of PI3K expression with representative blots (a), Akt activity (b), and cell death induction identified microscopically after staining with Hoechst 33342, acridine orange, and propidium iodide, respectively (c), in MOGGCCM and T98G cells. C—control; TR—cells incubated with transfection reagent only; TRsi—cells incubated with transfection reagent and siPI3K; TRsiT—cells incubated with transfection reagent, siPI3K, and temozolomide; * p < 0.01 compared to control.
