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. 2021 May 13;13(5):1637. doi: 10.3390/nu13051637

Figure 4.

Figure 4

Effect of FA on glucose metabolism in cultured MDA-MB-231 cells. MDA-MB-231 cells cultured in standard (2.2 µM) or FA-deficient (0 µM) medium for 3 weeks prior to analysis. Glucose uptake determined by 2NBDG staining (n = 3/group) (A). Expression of GLUT4 (B), LDHA (C), HK2 (D), H6PD (E), and PHGDH (F) determined by qPCR (n = 3/group). Cytotoxicity following withdrawal of FA in the presence of 50 mM 2-deoxyglucose (2DG), 30 µM 3-bromopryuvate (3BrPyr), or 10 µM polydatin (PolyD) for 48 h as determined by MTT assay (n = 4/group) (G). Student’s t-test (A–F) or one-way ANOVA (G) used to determine statistical significance (p < 0.05) between groups. Different letters indicate significant differences determined by Student’s t-test. Asterix’s indicate significant differences determined by one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).