Effect of the sequences of template and peptide chain on conversion of primer 2g in the anchoring of dipeptido dinucleotides (3gly2, 4, 5, or 6), as expressed in yields (%) after 6 h in condensation buffer at 0 °Ca,b.
| Template | Dipeptido dinucleotide | |||
|---|---|---|---|---|
| GlyGly-AA (3gly2) | LeuLeu-AA (4) | PhePhe-AA (5) | GlyPro-AA (6) | |
| — | 4 | 4 | 5 | 23 |
| 1cauu | 7 | 3 | 9 | 21 |
| 1cguu | 5 | <1 | 8 | 8 |
| 1ccuu | <1 | <1 | 7 | 10 |
| 1cuuu | 8 | 20 | 16 | 66 |
| 1cuuc | 28 | 82 ± 2c | 56 ± 3c | Quantd |
a
As detected by MALDI-TOF MS; a correction factor of 1.1 was applied to compensate for differences in desorption/ionization; see the ESI for details.
b
Conditions: 20 μM DNA 2g, 20 μM template, 200 μM dipeptido dinucleotide, 50 mM MOPS buffer, pH 7.4, 0.2 M EDC, 0.2 M NaCl, 0 °C.
c
Mean ± one standard deviation from four independent assays.
d
Quantitative conversion.