Kinetics of anchoring reactions involving dipeptido dinucleotides 4–9, primer 2g, and different RNA templates, as obtained from fits^a,b.

Template	Peptido dinucleotide	k (h−1)	t 1/2 (h)	y max (%)
1cuuc	4	0.39 ± 0.03	1.8	93 ± 3
1cuuc	5	0.24 ± 0.02	2.9	80 ± 1
1cuuc	6	0.91 ± 0.06	0.76	86 ± 2
1cguu	7	0.031 ± 0.005	22	30 ± 2
1cguu	8	0.087 ± 0.007	8.0	48 ± 1
1cguu	9	0.087 ± 0.003	8.0	50 ± 0.4

^aFits to: y = y_max (1 − e^−kt), where y is the yield, k is the rate constant, and t_1/2 is the half-life time for formation of the POR. Error values are those obtained by fitting with OriginPro, version 8. See Fig. S17–S36 of the ESI for spectra and kinetic plots.

^bConditions: 20 μM DNA primer, 20 μM template, 200 μM dipeptido dinucleotide, 50 mM MOPS buffer, pH 7.4, 0.2 M EDC, 0.2 M NaCl at 0 °C.