Table 1.
Materials and reagents
| Materials | Resource | Identifier |
|---|---|---|
| Mice (shortname) | ||
| C57BL/6J (wild type) | The Jackson Laboratory | IMSR_JAX:000664 |
| C57BL/6.FVB-Tg(Slc6a5-EGFP)13Uze (GlyT2::eGFP) | IPT (Zurich, Switzerland) | MGI:3835459, Zeilhofer et al. (2005) |
| Cck<tm1.1(cre)Zjh>/J (CCKCre) | Jackson Laboratory | Taniguchi et al. (2011) |
| Viral vectors short name | ||
| rAAV-retro/2-shortCAG-dlox-EGFP | VVF (Zurich, Switzerland) | This publication (vHW22-retro) |
| rAAV-retro/2-shortCAG-tdTomato | VVF (Zurich, Switzerland) | v131-retro |
| rAAV-retro/2-hEF1a-DreO | VVF (Zurich, Switzerland) | v127-retro |
| ssAAV-DJ/2-hEF1a-DreO | VVF (Zurich, Switzerland) | v127-DJ |
| rAAV-9/2-hEF1α-Don/Con-EGFP | VVF (Zurich, Switzerland) | This publication (vHW18–1) |
| rAAV-retro/2-hCMV-Cre | VVF (Zurich, Switzerland) | v36-retro |
| rAAV1-CAG-flex.eGFP | Penn Vector Core (Philadelphia, USA) | V3675TI-Pool |
| rAAV-8/2-hSyn1-roxSTOP-dlox-TVA_2A.RabG | VVF (Zurich, Switzerland) | This publication (vHW7–1) |
| SAD.RabiesΔG.eGFP (EnvA) (EnvA.RV.dG.eGFP) | Salk Institute (La Jolla, CA, USA) | Albisetti et al. (2017) |
| AAV1-CAG-flex-tdTom | Penn Vector Core (Philadelphia, USA) | AllenInstitute854 |
| rAAV2-EF1α-flex-WGA | IPT (Zurich, Switzerland) | This publication |
| ssAAV-1/2-hSyn1-dlox-mSyp1-EGFP | VVF | vHW41.1 |
| Primary antibodies (dilution) | ||
| Goat anti-Pax2 (1:400) | R&D Systems (Minneapolis, MN, USA) | AB_10889828 |
| Guinea pig anti-Lmx1b (1:10 000) | Dr Carmen Birchmeier | Muller et al. (2002) |
| Chicken anti-GFP (1:1000) | Life Technologies (Carlsbad, CA, USA) | AB_2534023 |
| Rabbit anti-GFP (1:1000) | Molecular Probes (Eugene, OR, USA) | AB_221570 |
| Rabbit anti-NeuN (1:1000) | Abcam (Cambridge, UK) | AB_10711153 |
| Guinea pig anti-NeuN (1:1000) | Synaptic System (Göttingen, D) | AB_2619988 |
| Goat anti-WGA (1:2000) | VECTOR laboratories (Burlingame, CA, USA) | AS-2024 |
| Rabbit anti-WGA (1:2000) | Sigma Aldrich (Saint-Louis, MO, USA) | T4144 |
| Rabbit anti-c-Maf (1:1000) | Dr Carmen Birchmeier | #40 |
| Guinea pig anti-c-Maf (1:1000) | Dr Carmen Birchmeier | #2223, #1 Final bleed |
| Rabbit anti-PKCg (1:1000) | Santa Cruz (Dallas, Texas, USA) | AB_632234 |
| Rabbit anti-SST (1:1000) | Santa Cruz (Dallas, Texas, USA) | sc-13099 |
| Mouse anti-PV (1:1000) | Swant (Marly, Switzerland) | 235 |
| Guinea pig anti-PV (1:1000) | Immunostar (Hudson, WI) | 24428 |
| Rabbit anti-NPY (1:1000) | Peninsula Laboratories (San Carlos, CA, USA) | T-4069 |
| Rabbit anti-TVA | Dr Sauer | Seidler et al. (2008) |
| Goat anti-tdTomato (1:1000) | Sicgen (Cantanhede, Portugal) | AB8181-200 |
| Rabbit antihomer | Synaptic System (Göttingen, D) | AB_2120990 |
| Guinea pig antihomer | Synaptic System (Göttingen, D) | AB_10549720 |
| RNAscope multiplex FISH probes | ||
| CCK | ACD | Mm-CCK-C1 |
| CRE | ACD | CRE-C3 |
| GFP | ACD | Mm-GFP-C3 |
| RORα | ACD | Mm-Rora-C2 |
| Crhr1 | ACD | Mm-Crhr1-C2 |
| Etv1 (Er81) | ACD | Mm-Etv1-O1-C1 |
| Bcl11b (Ctip2) | ACD | Mm-Bcl11b-C1 |
| Htr2c (5-HTR2c) | ACD | Mm-Htr2c-C1 |
| GABARγ1 | ACD | Mm-Gabrg1-C1 |
| Plxnd1 | ACD | Mm-Plxnd1 |
| Nr4a2 (Nurr1) | ACD | Mm-Nr4a2-C1 |
| Triplex positive control probe | ACD | 3-plex Positive Control Probe- Mm |
| Triplex negative control probe | ACD | 3-plex Negative Control Probe- Mm |
Notes: IPT: Institute of Pharmacology and Toxicology, University of Zurich; VVF: Viral Vector Facility (University of Zurich; www.vvf.uzh.ch)