Table 1.
(a) | ||||||||||||
Family 1 | Family 2 | Family 3 | ||||||||||
Patient ID | VI-1 | VI-2 | VI-3 | V-4 | V-1 | V-9 | IV-1 | V-1 | V-2 | IV-1 | IV-2 | IV-3 |
Gene 1 | ||||||||||||
Gene name | ASPM | ASPM | ASPM | ASPM | ASPM | ASPM | ASPM | ASPM | ASPM | ASPM | ASPM | ASPM |
Zygosity | Homo | Homo | Homo | Homo | Homo | Homo | Homo | Homo | Homo | Homo | Homo | Homo |
cDNA mutation | c.9601C>T | c.9601C>T | c.9601C>T | c.9601C>T | c.9601C>T | c.9601C>T | c.719_720delCT | c.719_720delCT | c.719_720delCT | c.9492T>G | c.9492T>G | c.9492T>G |
Protein mutation | p.(Gln3201*) | p.(Gln3201*) | p.(Gln3201*) | p.(Gln3201*) | p.(Gln3201*) | p.(Gln3201*) | p.(Ser240Cysfs*16) | p.(Ser240Cysfs*16) | p.(Ser240Cysfs*16) | p.(Tyr3164*) | p.(Tyr3164*) | p.(Tyr3164*) |
Gene 2 | ||||||||||||
Gene name | - | - | - | - | WDR62 | WDR62 | - | CEP63 | CEP63 | RAD50 | RAD50 | - |
Zygosity | - | - | - | - | Hetero | Hetero | - | Hetero | Hetero | Hetero | Hetero | - |
cDNA mutation | - | - | - | - | c.3116A>G | c.3116A>G | - | c.1241A>T | c.1241A>T | c.3643C>G | c.3643C>G | - |
Protein mutation | - | - | - | - | p.(Ser1106Gly) | p.(Ser1106Gly) | - | p.(Thr421Ser) | p.(Thr421Ser) | p.(Leu1215Val) | p.(Leu1215Val) | - |
Measurement | ||||||||||||
Age (years) | 12 | 9 | 6 | 23 | 32 | 10 | 34 | 6 | 3 | 18 | 14 | 10 |
Gender | Male | Female | Female | Male | Female | Male | Female | Male | Female | Female | Female | Female |
HC (cm) | 41 | 35 | 34 | 42 | 41.5 | 37 | 35 | 37.5 | 32 | 41 | 43 | 38 |
HC (SD) | −10 | −14.55 | −15 | −10 | −10 | −12 | −14.55 | −11 | −15 | −10.5 | −9 | −13 |
Height (cm) | 133 | 115 | 105 | 162 | 124 | 99 | 145 | 93 | 76 | 137 | 145 | 112 |
Height (SD) | −2 | −3 | −2.5 | −2 | −7 | −6 | −3 | −5 | −5 | −5 | −3 | −4 |
Neurological features | ||||||||||||
ID | Moderate | Mild | Severe | Moderate | Severe | Mild | Moderate | Moderate | Moderate | Severe | Severe | Mild |
Behavior | Aggressive | Aggressive | Aggressive | Aggressive | Aggressive | Aggressive | Aggressive | Aggressive | Aggressive | Aggressive | Aggressive | Normal |
Speech impairment | Severe | Severe | Severe | Severe | Severe | Severe | Severe | Moderate | Severe | Mild | Mild | Mild |
Musculoskeletal abnormalities | ||||||||||||
Contractures | - | - | - | - | Joints (elbow and hands) | Joints (elbow and hands) | - | - | - | - | - | - |
Clinodactyly of toes | - | - | - | - | - | - | - | - | - | - | - | - |
Clinodactyly of fingers | - | - | - | - | - | - | - | - | - | - | - | - |
Brachydactyly of fingers | - | - | - | - | - | - | - | - | - | - | - | - |
Brachydactyly of toes | - | - | - | - | - | - | - | - | - | - | - | - |
Others | ||||||||||||
Drooling | - | - | - | - | Yes | Yes | - | - | - | - | - | - |
Locomotion | Normal | Normal | Normal | Normal | Normal | Normal | Normal | Hyperactive | Hyperactive | Normal | Normal | Normal |
Seizures | - | - | - | - | - | - | - | Yes | Yes | Yes | Yes | - |
Hypopigmentation | - | - | - | - | - | - | - | - | - | - | - | - |
Teeth | Normal | Normal | Normal | Normal | Normal | Normal | Normal | Normal | Normal | Normal | Normal | Normal |
(b) | ||||||||||||
Family 4 | Family 5 | |||||||||||
Patient ID | V-6 | V-7 | V-8 | VI-2 | VI-3 | VI-4 | IV-1 | IV-2 | ||||
Gene 1 | ||||||||||||
Gene name | CENPJ | CENPJ | CENPJ | CENPJ | CENPJ | CENPJ | CENPJ | CENPJ | ||||
Zygosity | Homo | Homo | Homo | Homo | Homo | Homo | Homo | Homo | ||||
cDNA mutation | c.3586G>A | c.3586G>A | c.3586G>A | c.3586G>A | c.3586G>A | c.3586G>A | c.3586G>A | c.3586G>A | ||||
Protein mutation | p.(Asp1196Asn) and p.(Val1181_Val1206del) | p.(Asp1196Asn) and p.(Val1181_Val1206del) | p.(Asp1196Asn) and p.(Val1181_Val1206del) | p.(Asp1196Asn) and p.(Val1181_Val1206del) | p.(Asp1196Asn) and p.(Val1181_Val1206del) | p.(Asp1196Asn) and p.(Val1181_Val1206del) | p.(Asp1196Asn) and p.(Val1181_Val1206del) | p.(Asp1196Asn) and p.(Val1181_Val1206del) | ||||
Gene 2 | ||||||||||||
Gene name | - | - | - | PCNT | PCNT | PCNT | - | - | ||||
Zygosity | - | - | - | Hetero | Hetero | Hetero | - | - | ||||
cDNA mutation | - | - | - | c.5767C>T | c.5767C>T | c.5767C>T | - | - | ||||
Protein mutation | - | - | - | p.(Arg1923*) | p.(Arg1923*) | p.(Arg1923*) | - | - | ||||
Measurement | ||||||||||||
Age (years) | 32 | 22 | 20 | 8 | 6 | 4 | 10 | 4 | ||||
Gender | Female | Male | Female | Male | Male | Female | Male | Male | ||||
HC (cm) | 44 | 47 | 45 | 39 | 35 | 35 | 35 | 35 | ||||
HC (SD) | −8.5 | −7 | −7.5 | −10 | −13 | −11.5 | −12 | −11 | ||||
Height (cm) | 139 | 156 | 145 | 107 | 91 | 86 | 114 | 81 | ||||
Height (SD) | −4.5 | −4 | −4 | −4 | −5 | −4 | −4 | −5 | ||||
Neurological features | ||||||||||||
ID | Moderate | Moderate | Moderate | Mild | Mild | Mild | Moderate | Moderate | ||||
Behavior | - | - | - | - | - | - | Normal | Aggressive | ||||
Speech impairment | Severe | Moderate | Mild | Moderate | Severe | Moderate | Moderate | Moderate | ||||
Musculoskeletal abnormalities | ||||||||||||
Contractures | - | - | - | - | - | - | - | - | ||||
Clinodactyly of toes | - | - | - | Yes (bilateral) | Yes (bilateral) | Yes (bilateral) | - | - | ||||
Clinodactyly of fingers | - | - | - | - | - | - | - | - | ||||
Brachydactyly of fingers | - | - | - | Yes (bilateral) | Yes (bilateral) | Yes (bilateral) | - | - | ||||
Brachydactyly of toes | - | - | - | Yes (bilateral) | Yes (bilateral) | Yes (bilateral) | - | - | ||||
Others | ||||||||||||
Drooling | - | - | - | Yes | Yes | Yes | Very rare | - | ||||
Locomotion | Normal | Normal | Normal | Normal | Normal | Normal | Normal | Normal | ||||
Seizures | - | - | - | - | - | - | Very rare | Normal | ||||
Hypopigmentation | Yes (forehead) | Yes (forehead) | - | - | - | - | - | - | ||||
Teeth | Normal | Normal | Normal | Normal | Normal | Normal | Malocclusion | Normal |
Note: “-” means absence of the feature; NR, No Record; HC, Head Circumference; ID, Intellectual Disability; cm, Centimeter; SD, Standard Deviation. Second mutation of CENPJ shown in family 4 and 5 is based on RT-PCR data, which revealed a second transcript lacking 78 bp (c.3541_3618del) of CENPJ and resulting in an in-frame deletion of 26 amino acids, p.(Val1181_Val1206del).