Fig. 2. SDS-PAGE of VFe proteins and densitometry data. Panel A. Shown is the molecular weight ladder (lane L) with masses in kDa on the left, VFeStr protein prepared from wild-type (DJ2253) (lane 1), apo-VFe protein from a strain deleted for nifB (DJ2256) (lane 2). The positions of the different proteins are shown on the right. Panel B. Reported is the ratio of protein concentrations taken from densitometry scans of Panel A for VFeStr protein α-(VnfD) and β-(VnfK) subunits and copurifying proteins prepared from the wild-type (WT) (DJ2253) and ΔnifB strain (DJ2256).
