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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Brain Behav Immun. 2020 Jun 11;89:184–199. doi: 10.1016/j.bbi.2020.06.016

Fig. 4. Differential expression of chemokines in LCN2 deficient mice.

Fig. 4.

(A-F) Expression of mRNA in cerebral cortex of 9 months-old mice was analyzed by qRT-PCR as described in Materials and Methods. Relative expression was calculated using the 2-ΔΔCT method. (G-K) Protein was extracted from cerebral cortices of 9–10 months old animals and 60 μg per sample were used to determine levels of soluble chemokines using a multiplex assay as described in Materials and Methods. Note that Milliplex Analyst 5.1 returned CCL4 values for all WT as 19.74 pg/ml which is the lower detection limit for this factor. Values are mean ± s.e.m.; n = 6 (female n = 3; male n = 3) per genotype; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001, ANOVA followed by Fisher’s PLSD post hoc test.