Table 1.

Summarized characteristics of isolated yeast strains (RSO, KTF, RBF), S. cerevisiae BY4742, S. var. boulardii (SB). The survival ability under different temperatures and pHs, growth in the presence of 0.3% bile salt, autoaggregation ability, galactose utilization and strain identification are shown. a–d: Different superscript letters in the same column indicate statistical differences in each strain at the level of p ≤ 0.05 as measured by Tukey’s test. All the values were represented as mean SDs of three independent replicates from at least two independent experiments. The symbols (√) or (-) indicated an ability or inability of isolates found under tested conditions, respectively.

No.	Isolate Name	Temp. 30 °C	Temp. 37 °C	Temp. 42 °C	PH 2.5	PH 2.0	0.3% Bile Salt	Auto- Aggregation Ability (%)	Galactose Utilization Ability	Genus/Species Identification
1	RSO1	✓	✓	✓	✓	✓	✓	100 a	✓	Saccharomyces cerevisiae
2	RSO2	✓	✓	✓	✓	✓	✓	100 a	✓	Saccharomyces cerevisiae
3	RSO3	✓	✓	✓	✓	✓	✓	100 a	✓	Saccharomyces cerevisiae
4	RSO4	✓	✓	✓	✓	✓	✓	98 a	✓	Saccharomyces cerevisiae
5	KTF1	✓	✓	-	✓	✓	✓	80 b	✓	Non- Saccharomyces
6	KTF2	✓	✓	-	✓	✓	✓	86 b	✓	Non- Saccharomyces
7	KTF3	✓	✓	✓	✓	✓	✓	91 a	✓	Meyerozyma guilliermondii
8	KTF5	✓	✓	✓	✓	✓	✓	95 a	✓	Meyerozyma guilliermondii
9	KTF6	✓	✓	✓	✓	✓	✓	73 c	✓	Non- Saccharomyces
10	KTF16	✓	✓	✓	✓	✓	✓	78 c	✓	Non- Saccharomyces, Meyerozyma
11	KTF18	✓	✓	✓	✓	✓	✓	72 c	✓	Meyerozyma guilliermondii
12	RBF19	✓	✓	-	✓	✓	✓	89 b	✓	Non- Saccharomyces
13	RBF20	✓	✓	-	✓	✓	✓	68 d	✓	Meyerozyma guilliermondii
14	RBF27	✓	✓	-	✓	✓	✓	81 b	✓	Non- Saccharomyces
15	RBF37	✓	✓	✓	✓	✓	✓	52 d	✓	Meyerozyma guilliermondii
16	RBF47	✓	✓	✓	✓	✓	✓	70 c	✓	Meyerozyma guilliermondii
17	RBF56	✓	✓	✓	✓	✓	✓	88 b	✓	Meyerozyma guilliermondii
18	BY4742	✓	-	-	✓	✓	✓	100 a	✓	Saccharomyces cerevisiae
19	SB	✓	✓	-	✓	✓	✓	96 a	-	Saccharomyces var. boulardii