Figure 4.

In vivo efficacy of the amide NPs and ester NPs compared with resiquimod with/without checkpoint blockade (aPD-1). Treatment protocol for preclinical studies with mice bearing pre-established bilateral NDL breast cancer tumors (N=4 animals per group) (A). The treatment began when tumor sizes were ca. 4 mm in diameter. Average percentage changes in tumor volumes ± standard deviations (pooled, average of all tumors per group per time point) (B, C) and the corresponding weight change (pooled) (D, E). Figures (C) and (E) are extracted from the selected areas in (B) and (D) respectively. Statistical significance was determined via one-way ANOVA (per time point) with a Tukey post-test (n.s = not significant, *p ≤ 0.05). Plots of the change in tumor volumes of individual tumors and body weights are available in SI (Figure S10).