Entry	A	A′		B	B′		A′ + B′
	Sequence	Sequence	%	Sequence	Sequence	%	%
20	H-SD′LSQRG-SAL ester		>98a, 48.0b	Boc-(Thz)(K–S)LSQRG-SAL ester		88.9b, 37.2d	84.3b, 50.4c
21				Boc-(Thz)NFS(K–S)QSNKRFLSKTQG-SAL ester		90.1b, 35.4d	89.9b, 45.8c

^aD′ = Asp(benzofuran), (K–S) = Lys–Ser dipeptide, SAL = salicylaldehyde.

^bPercentage conversion to the ligation product based on UPLC-MS analysis of the crude reaction mixture.

^cIsolated yield based on ligation.

^dIsolated yield over 2 steps (intramolecular STL and one-pot Thz deprotection).

^eIsolated yield over 2 steps (intramolecular STL and one-pot Fmoc deprotection).