Table 1.
Amplification efficiency and correlation coefficient of candidate reference genes.
| Gene | Amplification Efficiency (%) | Correlation Coefficient (R2) | Ct Value |
|---|---|---|---|
| ColGAPDH [22] | 106.14 | 0.9991 | 21.562 ± 0.865 |
| SsuACT | 99.17 | 0.9981 | 23.877 ± 1.059 |
| SsuCal7 | 106.74 | 0.9998 | 21.984 ± 0.619 |
| SsuCas | 104.35 | 0.9977 | 23.388 ± 1.467 |
| SsueIF5 | 103.76 | 0.9996 | 25.185 ± 0.807 |
| SsuGAPDH | 97.39 | 0.9996 | 20.608 ± 0.903 |
| SsuGTP | 100.02 | 0.9929 | 23.709 ± 4.902 |
| SsuHis | 109.7 | 0.9991 | 21.455 ± 1.150 |
| SsuMDH | 102.6 | 0.9994 | 20.113 ± 0.546 |
| SsuMet2 | 97.27 | 0.9995 | 22.875 ± 0.808 |
| SsuRIB | 94.92 | 0.9931 | 22.073 ± 1.003 |
| SsuTUA1 | 93.47 | 0.9991 | 25.556 ± 1.004 |
| SsuTUA2 | 104.27 | 0.9986 | 18.032 ± 0.984 |
| SsuTUB | 103.17 | 0.9984 | 22.314 ± 0.848 |
| SsuUBC1 | 105.46 | 0.9907 | 25.320 ± 0.817 |
| SsuUBC17 | 107.89 | 0.9993 | 21.644 ± 1.555 |
| SsuUBC2 | 104.03 | 0.9985 | 21.556 ± 1.681 |
| SsuUBCJ2 | 109.03 | 0.9975 | 24.512 ± 1.180 |
| SsuUDP | 108.71 | 0.9989 | 24.877 ± 1.782 |
Ct (cycle threshold) means the number of cycles experienced when the fluorescent signal in each reaction tube reaches the set domain value; SD: standard deviation. Ct values are mean ± SD (n = 3).