Table 2.
Semiquantitative analysis of relevant epithelial markers in control native UM (CTR) and in the stromal substitute of heterotypical substitutes of the human UM cultured in WM, EM, and PM media for 7 and 14 days as determined by immunofluorescence. Results were assessed as strong positive signal (+++), positive signal (++), mild signal (+), or negative (−).
Pancytokeratin | Cytokeratin 13 | Cytokeratin 7 | Cytokeratin 8 | Desmoplakin | Tight Junction Protein-1 | |
---|---|---|---|---|---|---|
CTR | +++ | +++ | +++ | +++ | +++ | +++ |
UM-WM 7D | ++ | − | − | + | + | − |
UM-WM 14D | ++ | − | − | + | + | − |
UM-EM 7D | + | − | − | − | + | ++ |
UM-EM 14D | + | + | + | + | + | ++ |
UM-PM 7D | ++ | − | − | ++ | + | − |
UM-PM 14D | ++ | − | − | + | ++ | − |