Figure 2.

(A,C,E) Box plots showing the log scale intensity of the 20 most intense ions in the m/z range of 600–1000, for individual power settings for each surgical instrument. The box represents the interquartile range with the median shown. The whiskers represent the range of data points, excluding outliers, which were defined as five times the standard deviation from the median value. Additionally, raw data are represented using jitter points. Raw data signal intensities (with no log scales) including minimum and maximum values for diathermy, laser, and harmonic were 787.8–27,923.8, 2911.9–14,049.5, and 3855.5–40,751.8, respectively. For spectral processing, background subtraction and lock mass correction using leucine enkephalin (m/z 554.2615) was applied. (B,D,F) PCA plots of the different power settings using the different surgical instruments in the m/z range of 600–1000. Ten distinct sampling points were made sequentially for each individual power setting and for each instrument during the same analytical session.