Table 2. Equine monoclonal antibodies against cell surface antigens and intracellular IL-4 used for staining of PBMC and flow cytometric analysis.
| Cell population | Antibody (clone) | Antigen | Fluorochrome/ Biotin | Reference | |
|---|---|---|---|---|---|
| i | Basophils | IgE (176) | Cell surface | A488 | [53] |
| MHCII (cz.11) | Cell surface | Biotin | [55] | ||
| IL-4 (13G7) | Intracellular | A647 | [56] | ||
| ii | T-cells | CD4 (HB61A) | Cell surface | A647 | [57] |
| CD8 (CVS8) | Cell surface | Biotin | [57] | ||
| IL-4 (13G7) | Intracellular | A488 | [56] | ||
| iii | B-cells | IgM (1–22) | Cell surface | A488 | [58] |
| IgG1 (CVS45) | Cell surface | Biotin | [59] | ||
| IL-4 (13G7) | Intracellular | A647 | [56] | ||
| iv | Monocytes | CD14 (105) | Cell surface | Biotin | [60] |
| IgE (176) | Cell surface | A488 | [53] | ||
| IL-4 (13G7) | Intracellular | A647 | [56] |