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. 2021 May 26;16(5):e0251601. doi: 10.1371/journal.pone.0251601

Fig 4. Characterization of immune suppressive potential of MSC, Treg, and Treg+MSC on activated rat splenocytes and human PBMC in vitro.

Fig 4

(A): TNF-a production (ELISA) by rat splenocytes after LPS stimulation. (B): IFNy production (ELISA) by rat splenocytes after ConA stimulation. (C-D): Pro-inflammatory cytokine production, TNFa (C) and IFNy (D) by activated human Donor 1 PBMC after anti-CD3/CD28 bead stimulation (ELISA). (E-F): Pro-inflammatory cytokine production, TNFa (E) and IFNy (F) by activated human Donor 2 PBMC after anti-CD3/CD28 bead stimulation (ELISA). All samples run in triplicate. Statistical significance between naive/treatment and activated control is indicated with (#) for p ≤ 0.05, (##) for p ≤ 0.01, (###) for p ≤ 0.001. Statistical significance between treatment groups is indicated with (*) for p ≤ 0.05, (**) for p ≤ 0.01, (***) for p ≤ 0.001. Treg, regulatory T cell; MSC, mesenchymal stromal cell; PBMC, peripheral blood mononuclear cells; TNFa, tumor necrosis factor alpha; IFNy, interferon gamma; ELISA, enzyme-linked immunosorbent assay.