Figure 5.

Activation of p65 NF-s–B, p38 MAP kinase, and ERK1/2 (p44/p42 MAP kinases) in chondrocytes and synovial fibroblasts by IL-17A, IL-17AF, and IL-17F (all 10 ng/ml) after 10 minutes stimulation. (A) Relative ratio of phosphorylated protein over total protein compared to baseline control (0 mins). Friedman test for each intracellular protein with Dunn’s multiple comparisons test. Mean ± SD. N=3. *p < 0.05. (B) Representative western blots for vinculin (loading control), phosphorylated, and total protein of each intracellular signalling protein. Full-size, uncropped western blots, including additional time points, can be found in Supplementary Figure 4 .