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(a) The Cryo-EM structure of Cas9 (PDB: 5Y368b) where the catalytic H840 was mutated to alanine to stop the reaction. The distance between the scissile phosphate and the Mg2+ is 4.8 Å, and the N863 faces away from the catalytic site. (b) Adjusted Cas9 structure that brings HIS840 (converted from alanine) and ASN863 close to the corresponding positions in the catalytic site of Endo VII with a 3.3 Å distance between the scissile phosphate and the Mg2+.
