Table 1.

Calculated Free Energy Profile for the Catalytic Reaction of T4 Endonuclease VII^a

ref				wild type				H43N
ΔGpt	Δg‡,NA	Δg‡	$\Delta g_{\text{obs }}^{‡}$	ΔGpt	Δg‡,NA	Δg‡	$\Delta g_{\text{obs }}^{{}^{‡}\text{WT}}$	ΔGpt	Δg‡,NA	Δg‡
10.93	33	43.93	43.93	2.75	20.40	23.02	21.48	17.47	26.05	41.12

^aFree energies (kcal/mol) of the proton transfer (PT) step, the nucleophilic attack (NA) step, and the total barrier in the reference solution, the wild type, and the H43N mutant of Endo VII as well as the observed barrier converted from k_cat from ref 30.