Figure 5.

Simplified network of miRNA-mRNA interactions in metastasis. (A) A simplified network is presented from 15 RNAs involved in the shortest pathway connecting 5 mRNAs and 65 RNAs within four functional categories related to metastasis. RNAs changed by the five main miRNAs are displayed by number(s) above the vertices in red (downregulation) or blue (upregulation), and in bold (p < 0.05) or plain (p $\ge$ 0.05) fonts. Red and blue edges correspond to positive and negative correlations, respectively. (B) Subcategories show the difference in Z-scores according to extrapelvic metastasis; subcategories were selected based on significant Z-scores in all patients using the log₂FC values of all 72 RNAs included in this network. * p < 0.05, ** p < 0.01, *** p < 0.001. (C) Biological functions associated with the 67 RNAs and five groups formed by primary miRNAs in the network are shown using boxplots. Statistical analysis was performed using the Wilcoxon rank-sum test or Kruskal–Wallis test. Downregulation and upregulation of RNAs refer to log₂FC < −1.5 and log₂FC > 1.5, respectively.