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. 2021 May 13;22(10):5183. doi: 10.3390/ijms22105183

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Qualitative proteomic analysis of the cargos of EVs of E and EVs of TCT of T. cruzi. (A) Western blot and differential protein profile of EVs of E and EVs of TCT using polyclonal anti-T. cruzi antibodies. (B) Venn diagram of specific and shared proteins of EVs of E and EVs of TCT. (C) Pie chart representing the percentage of proteins of EVs of E and EVs of TCT that belong to multigene families. (D) Western blot analysis for the confirmation of cruzipain, trans-sialidase [8] and prohibitin 2 (PHB2) in EVs of T. cruzi: (1) cruzipain in whole lysates of TCT; (2) cruzipain in EVs of TCT; (3) trans-sialidase (mAb 39) in whole lysates of TCT; (4) trans-sialidase (mAb 39) in EVs of TCT; (5) PHB2 in whole lysates of E; and (6) PHB2 in EVs of E. (E) Scatter plot representing Gene Ontology (GO) terms enrichment analysis of proteins of the EVs of E and EVs of TCT, categorized by molecular function (p ≤ 0.01).