Table 1.
DNA concentration of EBN samples extracted with five DNA extraction methods as measured by spectrophotometry.
| EBN | Description | DNA Concentration (ng/μL) † | ||||||
|---|---|---|---|---|---|---|---|---|
| Type | Species ‡ | Origin | Wizard | NucleoSpin | Qiagen | SDS | SDS/Qiagen | |
| 1 | Unprocessed | A. fuciphagus | * Segamat, Johor | 3.65 ± 0.21 b | 1.25 ± 0.35 c | 4.33 ± 0.33 ab | 1.10 ± 0.18 c | 4.60 ± 0.42 a |
| 2 | Unprocessed | A. fuciphagus | * Kapar, Selangor | 4.40 ± 0.54 a | 0.83 ± 0.05 b | 3.65 ± 0.61 a | 1.58 ± 0.15 b | 4.23 ± 0.72 a |
| 3 | Unprocessed | A. fuciphagus | * Nibong Tebal, Penang | 3.33 ± 0.13 ab | 0.45 ± 0.13 c | 3.23 ± 0.90 b | 1.35 ± 0.06 c | 4.43 ± 0.73 a |
| 4 | Unprocessed | A. fuciphagus | * Klang, Selangor | 3.55 ± 0.13 c | 0.60 ± 0.08 e | 4.08 ± 0.25 b | 1.35 ± 0.13 d | 4.78 ± 0.21 a |
| 5 | Unprocessed | A. fuciphagus | ** Sarikei, Sarawak | 3.80 ± 0.81 a | 0.60 ± 0.08 b | 4.50 ± 0.26 a | 1.10 ± 0.08 b | 4.45 ± 0.19 a |
| 6 | Unprocessed | A. fuciphagus | ** Gomantong Cave, Sabah | 2.23 ± 0.43 c | 0.50 ± 0.08 d | 3.75 ± 0.53 b | 0.70 ± 0.00 d | 4.98 ± 0.44 a |
| 7 | Unprocessed | A. fuciphagus | ** Baram, Sarawak | 5.03 ± 0.78 a | 1.23 ± 0.15 c | 3.38 ± 0.10 b | 1.83 ± 0.10 c | 4.65 ± 0.70 a |
| 8 | Unprocessed | A. maximus | ** Gomantong Cave, Sabah | 3.20 ± 0.87 b | 0.45 ± 0.13 d | 1.35 ± 0.26 cd | 1.65 ± 0.19 c | 5.18 ± 0.68 a |
| 9 | Unprocessed | A. maximus | ** Niah Cave, Sarawak | 3.30 ± 0.70 b | 0.68 ± 0.10 d | 1.55 ± 0.13 cd | 1.73 ± 0.15 c | 5.10 ± 0.75 a |
| 10 | Unprocessed | A. maximus | ** Niah Cave, Sarawak | 4.30 ± 0.61 a | 0.35 ± 0.06 c | 1.90 ± 0.46 b | 2.28 ± 0.56 b | 5.68 ± 1.08 a |
| 11 | Unprocessed | A. maximus | ** Subis Cave, Sarawak | 4.50 ± 0.60 a | 0.55 ± 0.06 c | 1.58 ± 0.22 b | 2.15 ± 0.21 b | 4.75 ± 0.49 a |
| 12 | Processed | A. fuciphagus | 2.55 ± 0.37 b | 0.53 ± 0.10 c | 4.28 ± 0.33 a | 0.73 ± 0.15 c | 4.18 ± 0.50 a | |
| 13 | Processed | A. fuciphagus | 2.95 ± 0.13 b | 0.30 ± 0.00 d | 1.53 ± 0.13 c | 1.40 ± 0.14 c | 4.50 ± 0.16 a | |
| Average | 3.59 ± 0.49 | 0.64 ± 0.11 | 3.01 ± 0.35 | 1.46 ± 0.16 | 4.73 ± 0.54 | |||
‡ A. fuciphagus, Aerodramus fuciphagus; A. maximus, Aerodramus maximus. † Values are mean ± standard deviation with n = 4 and different superscript letters in the same row indicate significantly different (p < 0.05). * Peninsular Malaysia; ** East Malaysia.