Table 2.
Evaluation of five different DNA extraction methods using simple additive weighting technique.
| DNA Extraction Method | Attribute/Measured Data | Overall Score | Rank | |||||
|---|---|---|---|---|---|---|---|---|
| dsDNA ‡ | Purity † | PCR † | Time ‡ | Simplicity Φ | Safety Φ | |||
| Weightage (Σ = 1) | 1/6 | 1/6 | 2/6 | 1/6 | 1/6 | 1/6 | ||
| Wizard | 0.08 | 0.31 | 0.54 | 2.0 | 2 | 2 | 1.02 | 3 |
| NucleoSpin | 0.34 | 0.00 | 0.85 | 2.0 | 2 | 2 | 1.06 | 2 |
| Qiagen | 0.22 | 0.46 | 1.00 | 2.0 | 2 | 2 | 1.25 | 1 |
| SDS | 0.81 | 0.00 | 0.85 | 4.5 | 1 | 1 | 0.78 | 4 |
| SDS/Qiagen | 0.44 | 0.00 | 0.92 | 4.0 | 1 | 1 | 0.75 | 5 |
‡ dsDNA concentration measured by fluorometry (ng/mL); handling time (hours). † Sampling fraction with purity between 1.7 and 2.0 or successful PCR amplification with intense bands, respectively. Φ Direct rating of procedure simplicity, 1, simple; 2, more simple, and reagents safety, 1, safe; 2, more safe.