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Preparation of pooled human platelet lysate (HPL). Fresh or expired platelet concentrates are exposed to freezing/thawing for platelet lysis. Ten units of platelet lysates are pooled in one bag and further portioned. After a second freeze/thaw step, the bags are centrifuged to deplete the platelet fragments. Final vials of pooled HPL are stored at least at −30 °C until use.
