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. 2021 May 13;22(10):5182. doi: 10.3390/ijms22105182

Figure 3.

Figure 3

Experimental validation of three identified circRNAs in psoriatic skin and HaCaT keratinocyte cells. (a) Validation of a well-known intergenic i-circRNA (CDR1as), a novel exon i-circRNA (hsa_skin_052271), and a canonical circRNA (hsa_skin_088763) by PCR. The divergent and convergent arrows above the gel image represent, respectively, the divergent and convergent PCR primers used in RNA (cDNA) and DNA (gDNA), and the white arrows in the gel image point to circRNAs. Numbers on the left ranging from 100 to 1kb+ represent sizes in DNA ladder. GAPDH was used as an internal control. (b) Validation of CDR1as, hsa_skin_052271, and hsa_skin_088763 by Sanger sequencing. (c) Box and whisker plot for RNase R validation of differential expression of CDR1as, hsa_skin_052271, and hsa_skin_088763 when PP vs. PN skin was compared. (d) Quantitative real-time PCR (qRT-PCR) validation of differentially expressed circRNAs hsa_skin_088763, CDR1as, and hsa_skin_052271 in PP vs. PN skin. (e) RNA-seq (hsa_skin2 of validation set) validation of differentially expressed circRNAs CDR1as, hsa_skin_052271, and hsa_skin_088763 in PP vs. NN skin.