. 2021 Apr 9;6(15):10054–10071. doi: 10.1021/acsomega.0c06345

Table 1. Comparison of NQO1 Activity of Colorectal (HCT-116) and Lung (A549) Carcinoma Cell Lines, Breast Cancer Cell Lines (T47D and MDA-MB-231), and Normal Platelets^a.

name of the cell line	NQO1 activity (μmol/min/mg protein)
platelets	0.47 ± 0.63
T47D	16.06 ± 2.02
MDA-MB-231	14.36 ± 6.50
HCT-116	152.79 ± 5.09
A549	243.24 ± 14.16

To determine the NQO1 activity, which is an indicator of functionally active Nrf2, protein lysates were collected as detailed in section Experimental Section and NQO1 activity was estimated. Analysis of the data showed that breast carcinoma cell lines T47D and MDA-MB-231 had very low NQO1 activity compared to colorectal carcinoma cell line HCT-116 (moderate activity) and lung carcinoma cell line (A549). Human platelets had very low NQO1 activity.

Table 1. Comparison of NQO1 Activity of Colorectal (HCT-116) and Lung (A549) Carcinoma Cell Lines, Breast Cancer Cell Lines (T47D and MDA-MB-231), and Normal Plateletsa.

Table 1. Comparison of NQO1 Activity of Colorectal (HCT-116) and Lung (A549) Carcinoma Cell Lines, Breast Cancer Cell Lines (T47D and MDA-MB-231), and Normal Platelets^a.